Fig. 4.

A Chemical structure of NMM (a) and gross morphology of Col-0 (WT), as1-1, and as2-1 plants grown on MS medium without and with NMM for 21 days (b). B Leaf phenotypes of plants grown in the absence (a, b, c) and the presence of 3 or 4 μM NMM (d, e, f) for 14 days (except panel f shows 19 days after sowing as2-1 plant). (a, d) WT plants, (b, e) as1-1 plants, (c, f) as2-1 plants. The inset in panel (f) shows enlarged filamentous leaves of an as2-1 plant. Red arrowheads in panels (d, e, f) show filamentous leaves. Scale bars = 1 mm. C Frequencies of plants with abnormal leaves and lethal phenotypes after growth on medium that contained G-quadruplex ligand NMM. Plants (n = 128) were used for each NMM treatment. The growth and leaf phenotypes on each plant were scored under the stereomicroscope