Table 1.
Frequencies of plants with abnormal leaves and lethal phenotypes after growth on medium that contained berberine
| Number of plants with pointed leaves | Number of plants with trumpet-like and filamentous leaves | Number of plants without true leaves | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Berberine (μM) | 0 | 12.5 | 20 | 25 | 0 | 12.5 | 20 | 25 | 0 | 12.5 | 20 | 25 |
| Col-0 (WT) | 0 ± 0 | 7 ± 9 | 8 ± 8 | 7 ± 7 | 0 ± 0 | 2 ± 1 | 3 ± 2 | 5 ± 6 | 0 ± 0 | 3 ± 2 | 11 ± 6 | 17 ± 14 |
| as1-1 | 0 ± 0 | 8 ± 6 | 8 ± 5 | 9 ± 6 | 0 ± 0 | 3 ± 3 | 13 ± 4 | 18 ± 10 | 0 ± 0 | 1 ± 1 | 20 ± 21 | 27 ± 23 |
| as2-1 | 0 ± 0 | 5 ± 1 | 6 ± 2 | 3 ± 3 | 0 ± 0 | 8 ± 7 | 35 ± 16 | 28 ± 16 | 0 ± 0 | 2 ± 3 | 14 ± 12 | 34 ± 21 |
Plants (n = 96) were used for each berberine treatment. Each value indicates the mean and the standard deviation of 4 batches of experiments
The trumpet-like or filamentous leaves on each plant were counted under the stereomicroscope at 18 days after sowing