Table 2.
Frequencies of plants with abnormal leaves and lethal phenotypes after growth on medium that contained coptisine
| Number of plants with pointed leaves | Number of plants with trumpet-like and filamentous leaves | Number of plants without true leaves | ||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Coptisine (μM) | 0 | 20 | 25 | 30 | 40 | 50 | 0 | 20 | 25 | 30 | 40 | 50 | 0 | 20 | 25 | 30 | 40 | 50 |
| Col-0 (WT) | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 3 | 2 |
| as1-1 | 0 | 1 | 0 | 1 | 1 | 1 | 0 | 8 | 16 | 21 | 27 | 21 | 0 | 1 | 0 | 0 | 7 | 29 |
| as2-1 | 0 | 2 | 0 | 3 | 1 | 0 | 0 | 16 | 37 | 42 | 42 | 37 | 0 | 1 | 1 | 3 | 11 | 30 |
Plants (n = 96) were used for each coptisine treatment
The filamentous or trumpet-like leaves on each plant were counted under the stereomicroscope at 18 days after sowing