Figure 1.
Age and strain effects on nicotine preference. Female and male early adolescent (pnd 24–35) (a), middle adolescent (pnd 36–47) (b), late adolescent (pnd 48–59) (c), and adult (pnd 60+) (d) C3H/Ibg and C57BL/6J mice were examined for nicotine preference (percentage of total daily fluid consumed from the nicotine bottle) in a 2 bottle choice test. Analysis indicated that there was a main affect of age (p = 1.2 × 10−5) but not strain or sex for nicotine preference. Within strain results showed that early (p = 0.007) and middle adolescent (p = 1.1 × 10−5) C3H/Ibg mice had reduced preference for nicotine relative to adults (e). In contrast, early adolescent C57BL/6J mice exhibited reduced preference for nicotine relative to middle adolescent (p = 0.018) and adult (p = 0.015) animals (f). Data represent mean ± SEM.
