Figure 1. Functional mGlu₅Rs are expressed in the postnatal EGL.

(A)DNA gels of RT-PCR for mGlu₅R (216 bp) from 10 cell-aspirates and 1 cell-aspirate or solution (sol.) only.(B and D) Pseudocolored images of Fluo 4/OGB-loaded GCPs before (top) and after (bottom) DHPG application (30 s, 100 μM) in control (B) and in the presence of 60 μM MTEP (D). Arrows indicate several cells that have DHPG-induced Ca²⁺ increase in control solution. The EGL is demarcated by white dotted lines. Scale bar = 10 μm. Intensity color scale bar: 0 to 255 (saturation). (C and E) Ca²⁺ activity traces for cells indicated with arrows in (B and D). Scale bar: 20 s/0.2 ΔF/F₀ with F, fluorescence and F₀, baseline fluorescence. (F) Mean (± sem) Ca²⁺ activity graphs illustrating DHPG responses in the absence and presence of MTEP or MPEP. n= 6 slices. (G) Diagram illustrating the analyzed area under the curve during the first 30 s of agonist application. (H) Bar graphs of the area under the Ca²⁺ responses for DHPG and CHPG at both age groups, P2–P3 and P7–P12.