Figure 3. mGlu5R inhibition promotes GCL survival in the EGL.
(A) Montage of TUNEL staining (black) along the entire folia V–VI interface in control (top) and MPEP-treated (bottom) conditions. Inset shows imaged area. Scale bar: 100 μm. (B) Confocal micrographs of TUNEL (green) and DAPI (red) staining in the area between folia V–VI in control (top) and MPEP-treated (bottom) sagittal sections. Inset shows imaged area. Scale bar: 50 μm. (C) Average number of TUNEL+ cells per mm3 and % TUNEL+ cells in the EGL of control (black) and MPEP-treated animals (orange). (D) Reconstructions of transversal cerebellar sections stained for TUNEL (black) and DAPI (cyan) from P3 control or CHPG-treated mice. Scale bar: 100 μm. (E) Average number of TUNEL+ cells per mm3 and % TUNEL+ cells in the EGL of control (control) and CHPG-treated animals (cyan). *p < 0.05.
