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. Author manuscript; available in PMC: 2013 Aug 1.
Published in final edited form as: Mol Microbiol. 2012 Jul 5;85(3):557–573. doi: 10.1111/j.1365-2958.2012.08127.x

Figure 2. Over-expressing BRG1 promotes C. albicans hyphal growth and biofilm formation.

Figure 2

A. The THE1-CIp10 control and tet-BRG1 (ICY171) strains were grown under yeast conditions (YPD at 28°C) with or without doxycycline. Over-expression of BRG1 stimulates the production of hyphae. Cell walls were visualized with calcofluor white and nuclei with DAPI.

B. The tet-NRG1 (SSY50-B) and tet-NRG1+tet-BRG1 (ICY175) strains were grown in hypha inducing conditions (YPD at 37°C). BRG1 over-expression restores the ability of the tet-NRG1 strain to filament in the absence of DOX. Cell walls were visualized with calcofluor white and nuclei with DAPI.

C. To examine biofilm formation, cells from the indicated strains were incubated in SD medium at 30°C for 24h in 96-well microtitre plates. The bottom of the wells was photographed after non-adherent cells were washed away. A dense mass of cells covering the bottom of the well is visible when biofilms formed.