Table 1.
Strains used in this study.
Strain | Parent | Genotvne1 | Reference |
---|---|---|---|
SC5314 | wild-type | (Gillum et al., 1984) | |
BCa23-3 | CAI4 | nrg1::hisG- URA3-hisG/nrg1::hisG | (Braun et al., 2001) |
THE1 | ade2::hisG/ade2::hisG ura3::λ imm434/ura3:: λ imm434 ENO1/eno1::ENO1-tetR-ScHAP4AD-3xHA-ADE2 | (Nakayama et al., 2000) | |
SSY50-B | THE1 | NRG1/nrg1:: URA3-tetO-NRG1 | (Saville et al., 2003) |
THE1-CIp10 | THE1 | RPS1/RPS1:: URA3 | (Bernardo et al.,2008) |
ICY171 | THE1-CIp10 | RPS1:: URA3/RPS1: :SAT1-tetO-BRG1 | This study. |
ICY175 | SSY50-B | RPS1/RPS1: :SAT1-tetO-BRG1 | This study. |
ICY194 | SC5314 | BRG1/brg1Δ: :SAT1-FLIP2 | This study. |
ICY318 | ICY194 | BRG1/brg1 Δ : :FRT | This study. |
ICY322 | ICY318 | brg1Δ::FRT/brg1Δ: :SAT1-FLIP | This study. |
ICY326 | ICY322 | brg1 Δ ::FRT/brg1 Δ ::FRT | This study. |
ICY330 | ICY326 | brg1 Δ ::FRT BRG1::SAT1-FLIP | This study. |
ICY197 | SSY50-B | BRG1/brg1 Δ ::SAT1-FLIP | This study. |
ICY200 | ICY197 | BRG1/brg1 Δ ::FRT | This study. |
ICY276 | ICY200 | brg1 Δ ::FRT/brg1 Δ ::SAT1-FLIP | This study. |
ICY277 | ICY276 | brg1Δ: :FRT/brg1Δ::FRT | This study. |
ICY314 | ICY277 | brg1 Δ ::FRT/BRG1::SAT1-FLIP | This study. |
ICY293 | THE1 | RPS1/RPS1: :SAT1-ACT1p-NRG1 | This study. |
ICY295 | ICY293 | RPS1:: URA3-tetO-BRG1 | This study. |
ICY281 | THE1 | NRG1/nrg1Δ:: URA3-tetO-lacZ | This study. |
ICY307 | ICY281 | RPS1/RPS1: :SAT1-tetO-BRG1 | This study. |
VY561 | ago1::FRT/ago1::FRT | (Bernsteinet al., 2012) |
The full genotype is that of the parental strain with additional modifications as indicated.
SAT1-FLIP denotes the SAT1 flipper cassette.