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. Author manuscript; available in PMC: 2013 Oct 10.
Published in final edited form as: Virology. 2012 Jun 28;432(1):120–126. doi: 10.1016/j.virol.2012.06.006

Figure 2. HPV16 E7 expression impedes cyclin B degradation during mitosis in primary human foreskin fibroblasts (HFFs).

Figure 2

HFFs with stable expression of empty vector (HFF C) or HPV16 E7 (HFF E7) were collected at different times after release from a double thymidine block. Shown here is a representative experiment; similar results were obtained in two additional experiments. (A) Western blot analysis of E7 expression in the stable HFF populations. (B) FACS analyses of G2/M cell populations are shown for HFF C and HFF E7 at different times after release from a double thymidine block. (C) Western blot analyses of pRB, pH3S10, histone H3, cyclin B and actin are shown. Cyclin B quantifications relative to actin are indicated above the cyclin B blot. Values are presented relative to the highest (1) in either HFF C or HFF E7. The bar graph below the actin blot shows the relative cyclin B levels normalized to actin, providing a basis for comparison of HFF C and HFF E7.