SDS-PAGE analysis of IL6(T23)-PE38KDEL. IL6(T23)-PE38KDEL was expressed and purified. SDS-PAGE (10%) showed that the target protein at approximately 56 kDa could be detected using Coomassie blue staining and immunoblotting. Lane 1, pellet from cell lysate induced with IPTG; lane 2, supernatant from cell lysate induced with IPTG; lane 3, middle molecular weight standard marker; lane 4, inclusion body protein; lane 5, purified IL6(T23)-PE38KDEL (5 μg); lane 6, immunoblot of 0.5 μg pure IL6(T23)-PE38KDEL using anti-PE monoclonal antibody.