Methylation status of the SFRP1 gene in representative (Nos. 1–5) bladder cancer specimens and normal tumor-adjacent tissues. M, methylated; U, unmethylated; N, normal tumor-adjacent tissues; T, tumor tissue. SFRP1 was methylated in 5 bladder cancer tissues, Nos. 1–5, but was unmethylated in the corresponding normal tumor-adjacent tissues. SFRP1, secreted Frizzled-related protein 1.