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Following treatment with the demethylating agent, 5′-aza-deoxycytidine acid (DAC, 1 μg/ml), the expression of SFRP1 protein in the bladder cancer cell lines T24 and 5637 was detected by western blotting. +, treated by DAC; -, not treated by DAC. Upon DAC treatment SFRP1 protein expression was detected in T24 and 5637 cells.
