Figure 4.

The effect of corticosteroids on IFN-γ-induced STAT1 activation. Western blots using cell lysates from NS (A), S (B) and COPD patients (C) AM treated with or without dexamethasone (1 µM) for 1 h before stimulation with IFN-γ (100 ng·mL⁻¹) for 0–60 min. Blots were probed with antibodies for Phospho-Y701 STAT1 and the loading control β-actin. Results presented are representative blots from n= 3 experiments for each subject group. Densitometry analysis of phosphorylated STAT1 (Y701) stimulated with IFN-γ for 10 min ± dexamethasone is shown for each subject group. Data are presented as mean P-Y701 (±SEM) normalized to β-actin loading control for n= 3 subjects for each group.