Figure 6.

Effect of a JAK inhibitor (JAKi) on the IFN-γ enhanced LPS response in AM. Cells from COPD (n= 3) and S (n= 6) were treated with JAK inhibitor 1 (1 µM) for 2 h before treatment with IFN-γ (100 ng·mL⁻¹) for 16 h before stimulation with LPS (1 µg·mL⁻¹) for 24 h. TNF-α (A) and IL-6 (B) levels were measured by elisa. Repeat measures anova results of P < 0.0001 were found for both TNF-α and IL-6. Results for Bonferroni post-test are shown for IFN + LPS versus IFN + LPS + JAKi; ***P < 0.001. Data presented as mean cytokine release ± SEM.