Figure 5.

Obesity attenuates neutrophil calcium flux in response to chemoattractants. Cellular calcium flux response to the CXC cytokine KC (25 ng/ml) was determined in mature bone marrow neutrophils isolated from genetically obese (db/db) (A) and diet-induced obese (B) mice and compared with lean control mice using Indo-1AM cytosolic dye-loading and flow cytometry. Three separate experiments were performed on db/db and diet-induced obese mouse isolated neutrophils and respective control mice; representative runs are shown for each source.