Table A2.

Summary of the input parameters and their estimates derived from 50 simulated data sets using equations A5 and A6. Eight simulated singlet concentration-response curves were generated per data set to partially mimic the replication used in the [³⁵S]GTPγS binding experiments. Simulated variability was as described in the Materials and Methods. Parameter estimates are presented as mean ± SD

	Emax/1000	pKa	logε	logχ1	logχ2
Input	16.3	0.48	1.48	−0.12	0.18
Output	16.2 ± 1.1	0.48 ± 0.05	1.49 ± 0.04	−0.13 ± 0.05	0.18 ± 0.03

	logm	basal/1000	KGDP	logs
Input	−0.07	0.70	−0.30	−0.11
Output	−0.07 ± 0.03	0.69 ± 0.07	−0.3 ± 0.04	−0.10 ± 0.03