Table 2.

Effect of the number of conditions per experiment on the estimates of the parameters of equation 6

Simulation	Emax	pKa	logε	logχ1	logχ2	logχ3	logχ4	logχ5	logn	basal
Input	1.50	9.70	1.48	−1.60	−1.30	−1.00	−0.70	−0.40	0.30	0.90
5 curves	1.49 ± 0.07	9.75 ± 0.12	1.36 ± 0.22	−1.49 ± 0.26	−1.21 ± 0.18	−0.94 ± 0.15	−0.66 ± 0.12	−0.37 ± 0.08	0.34 ± 0.10	0.90 ± 0.04
3 curves	1.50 ± 0.07	9.80 ± 0.18	1.27 ± 0.36		−1.13 ± 0.30		−0.62 ± 0.19	−0.35 ± 0.11	0.37 ± 0.15	0.90 ± 0.05
2 curves	1.51 ± 0.07	9.77 ± 0.19	1.33 ± 0.38		−1.18 ± 0.32			−0.37 ± 0.12	0.35 ± 0.17	0.90 ± 0.05

Ten simulated experiments with five curves per occasion were analysed. Curves were then deleted from these sets to leave three or two curves per experiment. Parameter estimates are presented as mean ± SD.