Table 3.
Simulation | Emax | pKa | logε | logχ1 | logχ2 | logχ3 | logχ4 | logχ5 | logm | basal |
---|---|---|---|---|---|---|---|---|---|---|
1. Input | 1.50 | 9.30 | 2.70 | −2.42 | −2.02 | −1.62 | −1.22 | −0.82 | −0.15 | 0.90 |
1. Estimate | 1.51 ± 0.09 | 9.34 ± 0.20 | 2.75 ± 0.16 | −2.45 ± 0.12 | −2.06 ± 0.11 | −1.65 ± 0.10 | −1.25 ± 0.10 | −0.85 ± 0.08 | −0.16 ± 0.04 | 0.90 ± 0.05 |
2. Input | 1.50 | 9.70 | 2.70 | −2.91 | −2.43 | −1.95 | −1.48 | −1.00 | 0.00 | 0.90 |
2. Estimate | 1.51 ± 0.10 | 9.72 ± 0.07 | 2.69 ± 0.18 | −2.89 ± 0.17 | −2.43 ± 0.16 | −1.95 ± 0.14 | −1.48 ± 0.13 | −1.00 ± 0.10 | 0.00 ± 0.04 | 0.88 ± 0.06 |
3. Input | 1.50 | 9.70 | 2.48 | −2.73 | −2.26 | −1.78 | −1.30 | −0.82 | 0.30 | 0.90 |
3. Estimate | 1.52 ± 0.09 | 9.69 ± 0.04 | 2.46 ± 0.13 | −2.72 ± 0.13 | −2.23 ± 0.13 | −1.77 ± 0.11 | −1.30 ± 0.08 | −0.83 ± 0.04 | 0.30 ± 0.04 | 0.89 ± 0.05 |
4. Input | 1.50 | 9.70 | 2.30 | −2.01 | −1.41 | −0.81 | −0.20 | 0.40 | 0.30 | 0.90 |
4. Estimate | 1.50 ± 0.10 | 9.71 ± 0.07 | 2.30 ± 0.14 | −2.01 ± 0.13 | −1.41 ± 0.12 | −0.81 ± 0.06 | −0.20 ± 0.03 | 0.40 ± 0.06 | 0.31 ± 0.04 | 0.90 ± 0.06 |
Equation 12 | Emax | pKa | logε | logχ1 | logχ2 | logχ3 | logχ4 | logχ5 | logk− | basal |
---|---|---|---|---|---|---|---|---|---|---|
Input | 1.90 | 10.00 | 2.00 | −2.52 | −2.22 | −1.92 | −1.60 | −1.30 | 0.40 | 0.95 |
Estimate | 1.89 ± 0.15 | 10.2 ± 0.9 | 2.04 ± 0.16 | −2.54 ± 0.15 | −2.27 ± 0.14 | −1.95 ± 0.13 | −1.63 ± 0.11 | −1.31 ± 0.10 | 0.3 ± 1.0 | 0.94 ± 0.05 |
Trimmed* | 1.89 ± 0.15 | 10.0 ± 0.3 | 2.04 ± 0.15 | −2.54 ± 0.14 | −2.27 ± 0.13 | −1.95 ± 0.12 | −1.63 ± 0.11 | −1.31 ± 0.09 | 0.5 ± 0.5 | 0.93 ± 0.05 |
In the case of equation 12, two of the simulations resulted in extremely aberrant estimates of the ligand binding parameters (pKa= 14.4, 13.7; logk-=−4.05, −3.53). These replicates were excluded from the summary statistics in the ‘Trimmed’ row.
Parameter estimates are presented as mean ± SD.