Table 5.
Summary of the parameters (derived from equation 1) of the concentration-response curves to CCL17 and CCL22 in the [35S]GTPγS binding assay at various GDP concentrations
| Agonist | GDP (mmol·L−1) | pEC50 | *Emax (103 ccpm) | basal (103 ccpm) | n |
|---|---|---|---|---|---|
| CCL17 | 0.3 | 8.92 ± 0.14 | 3.6 ± 0.5 | 10.2 ± 1.0 | 1.22 (0.68, 2.18) |
| CCL17 | 0.5 | 8.76 ± 0.10 | 4.4 ± 0.7 | 9.3 ± 0.8 | 0.75 (0.56, 1.01) |
| CCL17 | 1 | 8.86 ± 0.06 | 5.3 ± 0.3 | 7.4 ± 0.7 | 0.91 (0.68, 1.24) |
| CCL17 | 5 | 8.57 ± 0.05 | 5.8 ± 0.4 | 4.9 ± 0.4 | 0.88 (0.73, 1.06) |
| CCL17 | 12.5 | 8.36 ± 0.11 | 4.4 ± 0.6 | 3.3 ± 0.4 | 0.90 (0.78, 1.03) |
| CCL17 | 37.5 | 8.36 ± 0.12 | 2.7 ± 0.3 | 2.0 ± 0.2 | 0.92 (0.69, 1.24) |
| CCL17 | 50 | 8.46 ± 0.13 | 2.2 ± 0.3 | 1.6 ± 0.1 | 1.03 (0.88, 1.22) |
| CCL22 | 0.3 | 9.59 ± 0.05 | 5.5 ± 0.4 | 9.9 ± 1.0 | 0.95 (0.66, 1.36) |
| CCL22 | 0.5 | 9.43 ± 0.06 | 6.2 ± 0.7 | 9.2 ± 1.0 | 0.86 (0.64, 1.14) |
| CCL22 | 1 | 9.38 ± 0.11 | 8.4 ± 1.0 | 7.4 ± 0.8 | 0.82 (0.59, 1.15) |
| CCL22 | 5 | 9.19 ± 0.07 | 9.4 ± 0.7 | 4.6 ± 0.5 | 0.77 (0.66, 0.90) |
| CCL22 | 12.5 | 8.95 ± 0.08 | 9.0 ± 0.8 | 3.0 ± 0.5 | 0.79 (0.73, 0.86) |
| CCL22 | 37.5 | 8.76 ± 0.04 | 6.7 ± 0.3 | 1.8 ± 0.2 | 0.73 (0.65, 0.82) |
| CCL22 | 50 | 8.70 ± 0.04 | 6.0 ± 0.2 | 1.5 ± 0.2 | 0.75 (0.6, 0.95) |
*
Using equation 1, the upper asymptote of the curves is Emax+basal.
Values are the mean ± SEM of four duplicate determinations except for n, which is presented as geometric mean with 95% confidence interval in parentheses.