Table 1.

Binding parameters of [³H]-NMS and [³H]-CGP-12,177 in human bronchus and lung

	Region of the airway	Bmax (fmol·mg−1 of protein)	KD (pM)
[3H]-NMS binding sites	Segmental bronchus	97 ± 11*	220 ± 28
	Subsegmental bronchus	43 ± 3*	105 ± 26
	Lung parenchyma	71 ± 7*	130 ± 14
[3H]-CGP-12,177 binding sites	Segmental bronchus	47 ± 9*	100 ± 37
	Subsegmental bronchus	90 ± 10*	81 ± 30
	Lung parenchyma	320 ± 26*	98 ± 10

Saturation binding experiments with intact tissue segments were carried out at 4°C.

Bmax: binding density mg⁻¹ total protein of the segments.

Data represent means ± SEM of four to five experiments.

^*Significantly different from the other regions (P < 0.05).