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Effects of AICAR on kidney SIRT1 expression in rats with RI/R injury. The expression of SIRT1 and housekeeping protein α-tubulin, measured by Western blot (20 µg nuclear protein fraction per lane), are given in (A). Kidney SIRT1 expression measured as SIRT1/α-tubulin ratio is given in (B). Means ± SEM from three different runs are given, n= 4–5 per group in each gel. *P < 0.05 versus sham. For abbreviations, see Figure 1 legend.
