Figure 4.

JNK activation by noradrenaline in human prostate tissue. Samples of human prostate tissue were stimulated in vitro with noradrenaline (30 µM) for 0, 5, 10 or 20 min. All samples including the unstimulated (‘0 min’) were exposed for identical periods to experimental conditions and simultaneously shock frozen. The phosphorylation state of JNK was assessed by Western blot analysis with a phospho-specific antibody, and reflects JNK activity. (A) Representative Western blots for phosphorylated JNK and total JNK. (B and C) Results from densitometric quantification of all experiments (data are means ± SEM from independent experiments with tissues from n = 8 patients). *P < 0.02, **P < 0.01, ***P < 0.001 versus ‘0 min’.