Figure 2.
Overexpression of miR-8 makes flies bigger and insensitive to ecdysone effects of growth suppression. (A) Gradually increasing miR-8 progressively increases pupal size. (Top left) Picture of pupae whose genotypes are indicated. (Bottom left) Amounts of miR-8 in larval fat bodies in each of the indicated flies. (Right) Quantification of the pupal volumes in each of the genotypes. Approximately 20 pupae per genotype were measured. (B) Feeding larva with 20E (0.3 mg/mL) decreases final pupal size, while overexpression of miR-8 largely abolishes the suppressive effects of 20E feeding. The flies indicated as either CyOgfp/UAS-mir-8 or mir-8G4/UAS-mir-8 are siblings generated from the same parents and reared in the same vial. Relative repression folds by 20E feedings are compared between the genotypes indicated. Approximately 30 pupae per genotype were measured. (#) P < 1.6 × 10−4; (**) P < 0.01 compared with respective controls. Error bars denote SEM.