Figure 4.

Perturbation of USH expression impedes ecdysone-mediated growth regulation. (A) Heterozygous mutants of USH (ush^vx22/+) show significant resistance to the growth-suppressive effect of 20E feeding (0.3 mg/mL). The flies indicated as CyOgfp/+ or ush^vx22/+ are siblings generated from the same parents and reared in the same vial. Relative repression folds by 20E feedings are compared between the genotypes indicated. Approximately 44 pupae per genotype were measured. (B) Knockdown of EcR in USH-expressing tissues noticeably increases the adult weight, and this effect is impeded by overexpression of USH. More than 21 flies per genotype were measured. (**) P < 0.01; (*) P < 0.04 compared with respective controls. Error bars denote SEM.