Table 2.
The incorporation of radiolabelled fatty acids into the major phospholipids of M. hyorhinis.
| Tentative identification | Lipid phosphorus | Radioactivity (% of total) | ||
|---|---|---|---|---|
| μg/mg protein | % of total | [3H]-palmitate | [3H]-oleate | |
| SPM | 6.6 ± 1.5 | 33.5 ± 6.7 | 1.0 ± 0.5 | 0.0 ± 0.0 |
| PC | 2.4 ± 0.5 | 12.6 ± 2.0 | 40.0 ± 3.5 | 19.0 ± 2.8 |
| PG | 2.4 ± 0.6 | 12.4 ± 3.1 | 12.4 ± 1.1 | 20.7 ± 5.8 |
| CL | 8.4 ± 1.3 | 41.5 ± 6.4 | 46.6 ± 4.4 | 60.0 ± 8.8 |
M. hyorhinis cells were grown in the presence of either [3H]-palmitate or [3H]-oleate. Lipids were extracted by the method of Bligh and Dyer [18] and separated by TLC on silica gel plates (Kiesel-gel 60 HR, Merck, Darmstadt, Germany) developed at room temperature by a two-dimensional system described above. The lipid spots were scraped off the plates and analyzed for radioactivity. To determine phosphorus in the phospholipid spots, the method of Zhou and Arthur [20] was used. The results are the average of three independent experiments using different batches of cells. SPM: sphingomyelin; PC: phosphatidylcholine; PG: phosphatidylglycerol; CL: cardiolipin.