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. 2012 Aug 15;139(16):3032–3039. doi: 10.1242/dev.075812

Fig. 3.

Fig. 3.

wntD is expressed at the intersection of RTK signaling and Dorsal activity. (A-C′) Wild-type embryos, triple stained for wntD transcripts (green), Dl (red) and dpERK (blue). (A,A′) Sagittal cross-sections of a stage 4 embryo. At this stage, dpERK staining reflects the activity of the Torso pathway. White bars indicate domains of wntD expression. (B) Quantification of wntD expression (green), nuclear Dl (red) and dpERK (blue) in sagittal cross-sections of stage 4 wild-type embryos (n=14 embryos). Error bars indicate s.e.m. wntD is expressed at the junction of the two inputs and there is inverse correlation between the amounts of wntD and nuclear Dorsal. (C,C′) Cross-section views of a stage 6 wild-type embryo. At this stage, dpERK staining reflects MAPK/Erk activation that is dependent on EGFR signaling. wntD is expressed at the point of intersection of the domains of nuclear Dl and activated MAPK/Erk. (A,B) Embryos are oriented with anterior to the left and dorsal side upwards.