Fig. 9.

Suppression of replication-stress-induced Rad52 foci formation in brc1Δ cells results in lethal mitoses. (A) The same ethanol fixed cells as in Fig. 8 were stained with DAPI and aberrant mitotic figures were scored by microscopy (DAPI + DIC). Data are means ± s.d. for three populations of 100 cells. (B) The viability of cells grown at 25°C was determined by plating aliquots onto YES medium with plates incubated at 25°C for 5 days. The culture was then shifted to 36°C for 4 hours and viability determined using the same dilutions as for the 25°C culture. Alternatively, cells were arrested in 11 mM HU for 6.5 hours at 25°C, washed by filtration, and then incubated at 25°C or 36°C, followed by viability measurement as for the no HU cultures. Data are normalized to no HU cultures grown at 25°C, and are means ± s.d., n=3.