Figure 8.

Rv0652 induces the expression of macrophage surface molecules on RAW264.7 macrophages. RAW264.7 cells were cultured for 24 hr in the presence of Rv0652 (5 μg/ml), Ag85 (5 μg/ml), or LPS (100 ng/ml), and the expression of the surface markers MR, CD80, CD86 and MHC class II was analysed by flow cytometry. Data are representative of three experiments showing similar results. Mean fluorescence intensities (MFI) calculated from histograms are presented as means ± SD of three independent experiments. *P < 0·05 or **P < 0·01 or ***P < 0·001 versus untreated cells.