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. 2012 Jun;136(2):192–197. doi: 10.1111/j.1365-2567.2012.03569.x

Figure 1.

Figure 1

Nanoparticle tracking analysis (NTA) and immunoblotting analysis of microvesicles secreted by Jurkat and CEM cells. (a) Screenshots of NTA of 100-nm beads, serum-fee RPMI-1640, and post-10 000 g conditioned medium from Jurkat and CEM cells. Image size recorded by video is approximately 100 μm × 80 μm. (b) Size and particle distribution plots of 100-nm and 400-nm control beads, and post-10 000 g conditioned medium from Jurkat (peak at 76 nm) and CEM (peak at 83 nm) cells. (c) Immunoblot analysis of detergent cell lysates (lys) and exosome and microvesicles (exo, post-100 000 g pellets) of Jurkat and CEM cells.