Figure 4.

Immunodepletion of microvesicles and nanoparticle tracking analysis (NTA) of lipopolysaccharide (LPS) treated monocyte-derived dendritic cells (DC). (a) Jurkat supernatants were incubated with the indicated amounts of anti-human CD45 magnetic beads overnight, before NTA. Data represent samples measured in triplicate with mean and standard deviation plotted. The data are representative of three separate experiments. (b) Human peripheral blood monocytes cultured in interleukin-4/granulocyte–macrophage colony-stimulating factor for 5 days to generate DC were incubated overnight in serum-free medium containing 50 ng/ml LPS. Post-10 000 g supernatants were analysed by NTA.