Fig. 4.

Immunostaining for podoplanin, P- and N-cadherin of the fourth ventricle. The double immunostained section for podoplanin and P-cadherin is adjacent to the double immunostained section for podoplanin and N-cadherin. The immunostained section for podoplanin and P-cadherin was re-stained by the H-E staining which shows nodulus (No), cerebellar peduncle (CeP), and cerebellar lingual (CL). The fluorescence with anti-podoplanin (arrowheads) is found on ependymal cells and choroid plexus epithelial cells, and on the pia mater around the cerebellar lingual. The fluorescence with anti-P-cadherin (arrow) is found inside the choroid plexus, where anti-podoplanin did not react in the merged image. The fluorescence with anti-N-cadherin (arrow) is also found on ependymal cells, and on the pia mater around the cerebellar lingual, where anti-podoplanin reacted (arrowheads), but not on choroid plexus epithelia. Bar: 100 μm.