Fig. 5.

The laser-scanning confocal microscopy of immunostaining for podoplanin, P- and VE-cadherins on the choroid plexus and ventricle epithelia of the lateral ventricle. The upper, middle, and lower panels are the same sections. The immunostained sections were re-stained by the H-E staining, which shows choroid plexus (CP) and ependymal cells (arrowhead, yellow). In the immunostaining with anti-podoplanin and anti-P-cadherin, the fluorescence with anti-podoplanin is found on the choroid plexus epithelial cells (arrowheads, white) and ependymal cells (arrowhead, yellow) on the ventricle side both on the cell surface and in the cell–cell junctions, where the fluorescence with anti-P-cadherin is found on the choroid plexus epithelial cells (arrow) at the basement membrane side both on the cell surface and in the cell–cell junctions. The region reacting with anti-podoplanin did not coincide with the region reacting with anti-P-cadherin in the merged image. In the immunostaining with anti-podoplanin and anti-VE-cadherin, the fluorescence with anti-podoplanin is found on the choroid plexus epithelial cells (arrowheads) on the ventricle side both on the cell surface and in the cell–cell junctions, whereas the fluorescence with anti-VE-cadherin is found on the choroid plexus endothelial cells (arrows) both on the cell surface and in the cell–cell junctions. The region reacting with anti-podoplanin did not coincide with region reacting with anti-VE-cadherin in the merged image. In the immunostaining with anti-VE-cadherin and anti-P-cadherin, the region reacting with anti-VE-cadherin on the choroid plexus endothelial cells (arrowheads) did not coincide with the region reacting with anti-P-cadherin on the choroid plexus epithelial cells (arrows) in the merged image. Bar: 20 μm.