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. 2012 May;4(5):380–395. doi: 10.1002/emmm.201200215

Figure 2. Differentiation of DA neurons from PD patient-specific iPSC.

Figure 2

Ctrl-, ID-PD and LRRK2-PD iPSC were differentiated into DA neurons and analysed by immunofluorescence for expression of TUJ1 (green) and TH (red) at the end of the 30-day differentiation protocol.

A-C. All iPSC-derived neurons express TUJ1 and TH. Shown are representative images of differentiation experiments from Ctrl-iPSC (cell line SP11.1), ID-PD iPSC (cell line SP08.1) and LRRK2-PD iPSC (cell line SP06.2). Nuclei are counterstained with DAPI, shown in blue. Scale bars, 50 µm.

D. Quantitative analyses of cells stained positive for TUJ1 (left bars) or TUJ1 and TH (right bars). TUJ1-positive cells are represented as the percentage of total number of cells (stained with DAPI). Bars represent average with SEM as error bars. Data for CONTROL is the average of 4 iPSC lines, for ID-PD from 7 iPSC lines and LRRK2-PD from 4 iPSC. No significant differences were found in the ability of iPSC to generate neurons [F(2,12) = 0.953; p = 0.413] or DA neurons [F(2,12) = 0.679; p = 0.526] after 30 days of differentiation.