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. Author manuscript; available in PMC: 2012 Jul 24.
Published in final edited form as: Leukemia. 2008 Jan 24;22(4):783–790. doi: 10.1038/sj.leu.2405104

Figure 5.

Figure 5

GM-CSF expression is reduced in non-vaccinated patients with AML. (a) GM-CSF concentration in peripheral blood (12 AML, 10 CML and 30 HD) and bone marrow (12 AML plus CML and 6 non-myeloid malignancies) was evaluated by ELISA. GM-CSF concentration in each sample was calculated as a mean of three independent measurements performed in duplicate. (b) GM-CSF mRNA expression in peripheral blood of patients with myeloid leukemia (n = 12) and HD (n = 9) was evaluated by RT-PCR and subsequent PCR with GM-CSF RNA-specific primers. For both (a) and (b), the boxes are the column medians, 25 and 75th percentiles; whiskers are the largest and smallest values in each group. AML, acute myeloid leukemia; CML, chronic myeloid leukemia; HD, healthy donors; ELISA, enzyme-linked immunosorbent assay; GM-CSF, granulocyte-macrophage colony-stimulating factor; RT-PCR, reverse transcription-PCR.