Table 1.
Anti GM-CSF Igs in vaccinated patients by ELISA and western blot
| UPN | Diagnosis | Anti-GM-CSF IgG titer in ELISAa (RU ml−1) | Anti GM-CSF Igs in western blotb |
||
|---|---|---|---|---|---|
| IgG | IgA | IgM | |||
| P1 | AML | 3 | − | − | − |
| P2 | CML | 120 | ++ | − | − |
| P3 | AML | 3 | − | − | − |
| P4 | CML | 16 | − | − | − |
| P5 | CML | 48 | ++ | − | − |
| P6 | MDS | 15 | − | − | – |
| P7 | AML | 23 | − | + | − |
| P8 | AML | 20 | − | + | + |
| P9 | AML | 18 | − | − | − |
| P10 | AML | 21 | − | − | − |
| P11 | MDS | 121 | − | − | − |
| P13 | AML | 23 | + | − | + |
| P14 | MDS | 22 | − | N/D | N/D |
| P15 | CML | 192 | ++ | − | − |
| P16 | AML | 245 | +++ | +++ | +++ |
| P17 | CML | 40 | ++ | N/D | N/D |
| P18 | MDS | 48 | − | ++ | − |
| P19 | MDS | 59 | + | + | − |
| P20 | AML | 266 | N/D | N/D | N/D |
Abbreviations: AML, acute myeloid leukemia; CML, chronic myeloid leukemia; ELISA, enzyme-linked immunosorbent assay; GM-CSF, granulocyte-macrophage colony-stimulating factor; Igs, immunoglobulins; RT-PCR, reverse transcription-polymerase chain reaction; MDS, myelodysplastic syndrome.
a
ELISA was performed for anti-GM-CSF IgG only.
b
The number of (+) reflects the band's intensity on western blot, (−) no visible band on western blot, (N/D) western blot was not performed because the limited quantity of serum.