Figure 3.

Gp74 also digests lambda phage DNA in the presence of Zn²⁺. Agarose gel (1%) electrophoresis analysis of the time course of the lambda phage DNA digestion by gp74 in the presence of 0.5–5 mM Zn²⁺ ions. As with the Ni²⁺ digestion experiments, the concentration of λ DNA was 50 μg/mL and that of gp74 was 48 μg/mL. Reactions were performed in 20 mM HEPES, pH 7.0. As seen in the presence of Ni²⁺, gp74 mediates digestion of λ DNA at multiple sites (smear) in the presence of Zn²⁺, albeit with reduced efficiency.