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Summary of Parameters Resolved From the First Transition of the Thermal-Unfolding Experiments for Wild-Type and X-Enabled NCAD12 in the Absence and the Presence of Calcium
| Protein | Buffer | ΔHm (kcal mol−1) | Tm (°C) | ΔG° at 25°C (kcal mol−1) |
|---|---|---|---|---|
| Wild-type | Apo | 68 ± 8 | 45 ± 2 | 3.6 ± 0.5 |
| Ca2+ | 79 ± 5 | 59 ± 3 | 6.3 ± 0.7 | |
| X-enabled | Apo | 69 ± 5 | 44 ± 1 | 3.6 ± 0.3 |
| Ca2+ | 82 ± 5 | 57 ± 1 | 6.2 ± 0.9 |
