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Experimental Kinetic Values for WT HIV-1 Protease and Specificity Designed Pr3 Variant for the Cleavage of Three Peptide Substrates
| RT-RH | p2-NC | CA-p2 | ||||
|---|---|---|---|---|---|---|
| Protease | Vmax/KM (s−1) | Normalized | Vmax/KM (s−1) | Normalized | Vmax/KM (s−1) | Normalized |
| Wild-type | 1.65E−03 | 1.00 | 3.70E−04 | 0.22 | 1.34E−03 | 0.81 |
| Pr3 | 1.13E−03 | 1.00 | 1.00E−04 | 0.09 | 1.00E−04 | 0.09 |
