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MS/MS spectra of (a) AVHLPSGGQYK (m/z = 386.2) and (b) the corresponding deamidated form of the peptide (m/z = 386.5). Spectra were obtained from separated HPLC peaks of a tryptic digest from an 88-year-old human lens. (c) The theoretical y- and b-ion fragments of AVHLPSGGQYK from γS crystallin. Masses were generated using the molecular weight calculator (http://ncrr.pnl.gov/software/).
