Figure 1.

Western blot to detect protein expression in A. aceti strain 1023 cell lysates, using a primary antibody raised against a peptide with the UctB N-terminal sequence. The bands visualized correspond to UctB (two closely spaced bands at 47 and 48 kDa) and an unidentified 59 kDa protein. Lanes marked with an L are from the first logarithmic growth phase, associated with the conversion of ethanol to acetic acid. Lanes marked with an S were harvested during the first stationary phase. The initial ethanol level of the YPDE cultures is indicated above each pair of lanes. A lane containing pure H6UctB (2 kDa larger than native UctB) was used as a standard. Each lane was loaded with 5 μg total protein. M indicates the position of size standards (in kDa).