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Reactions catalyzed by BacD [(A) and (C)] and Ddl (B). A: The physiological substrates of BacD are considered to be l-alanine and l-anticapsin. B: The previously proposed reaction scheme for Ddls consists of three steps. C: The phosphinate l-alanyl-l-phenylalanine analog (left) binds the active site of BacD, and accepts a phosphate group from ATP to form the phosphorylated phosphinate l-alanyl- l-phenylalanine analog (right, termed “P-analog” in this study).
