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. Author manuscript; available in PMC: 2012 Jul 24.
Published in final edited form as: J Biomol Screen. 2009 Jun 25;14(7):824–837. doi: 10.1177/1087057109338517

Figure 2.

Figure 2

Confirmation of primary TNAP hits. Cherry-picked hits were tested at 20 uM concentration in the luminescent (A and B) and colorimetric (C and D) assays. The concentration of DEA was 100 mM (A) or 1M (B, C, and D). Activity of the enzyme in the colorimetric assays was measured using absorbance of either p-nitrophenyl (C) or malachite green-phosphomolybdate complex (D). Experimental data was converted to % inhibition according to Eq. 1 using control values of the assay. Average values of each set of quadruplicates were calculated and utilized for each compound.