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Mode of inhibition studies for primary HTS hits in respect to CDP-star substrate. TNAP inhibition with MLS-0005718 (A), MLS-0039961 (B), MLS-0067142 (C), and MLS-0038949 (D) was measured in the presence of 1 uM (squares), 50 uM (triangles) or 300 uM (inversed triangles) CDP-star. The concentration of DEA was equal 100 mM.
