Table 2.
Effects of Arisolochiae manshuriensis Kom. (AMK) on the chromosomal aberration in Chinese hamster ovary (CHO)-K1 cells.
| Treatment (μg/mL) | AA I/ II (μg/mL) | S9 mix | Time (h)a | Aberrant metaphases (−Gap/+Gap) | PP + ER | RCCb (%) | |
|---|---|---|---|---|---|---|---|
| Vehicle control | − | − | 6–18 | 0.0/0.0 | 0.0 | 100.0 | |
|
| |||||||
| 625 | 1.78/0.31 | − | 6–18 | 1.0/1.5 | 0.0 | 86.5 | |
| AMK | 1,250 | 3.56/0.63 | − | 6–18 | 0.5/0.5 | 0.0 | 77.8 |
| 2,500 | 7.13/1.25 | − | 6–18 | 2.0/2.0 | 0.0 | 54.6 | |
|
| |||||||
| MMC | 0.04 | − | − | 6–18 | 22.0/22.5∗ | 0.0 | − |
| 0.0 | |||||||
|
| |||||||
| Vehicle control | − | + | 6–18 | 0.5/0.5 | 0.0 | 100.00 | |
|
| |||||||
| 1,250 | 3.56/0.63 | + | 6–18 | 0.5/0.5 | 0.0 | 92.1 | |
| AMK | 2,500 | 7.13/1.25 | + | 6–18 | 0.5/1.0 | 0.0 | 86.2 |
| 5,000 | 14.25/2.50 | + | 6–18 | 1.0/1.5 | 0.0 | 69.1 | |
|
| |||||||
| CPA | 10.00 | − | + | 6–18 | 27.0/27.5∗ | 0.0 | − |
| 0.0 | |||||||
|
| |||||||
| Vehicle control | − | − | 24–0 | 0.5/0.5 | 0.0 | 100.0 | |
|
| |||||||
| 625 | 1.78/0.31 | − | 24–0 | 0.5/1.0 | 0.0 | 75.1 | |
| AMK | 1,250 | 3.56/0.63 | − | 24–0 | 1.5/2.0 | 0.0 | 72.5 |
| 2,500 | 7.13/1.25 | − | 24–0 | 2.5/2.5 | 0.0 | 53.9 | |
|
| |||||||
| MMC | 0.04 | − | − | 24–0 | 27.0/28.0∗ | 0.0 | − |
Abbreviations: PP: polyploidy; ER: endoreduplication; RCC: relative cell counts; MMC: mitomycin C; CPA: cyclophosphamide.
aTreatment time-recovery time.
bRCC (relative cell count) equals (no. of treated cells/no. of control cells) × 100 (%).
∗Significantly different from the vehicle control at P < 0.05.