Figure 1.

Differential extraction of Angelica sinensis (AS).   The powder of AS was extracted sequentially with acetone, methanol, and water. The extracts were concentrated under reduced pressure to yield an acetone extract (AS-AE), methanol extract (AS-ME), and water-soluble extract (AS-WE). The layer of AS-ME was chromatographed on an Amberlite XAD-4 column and gave 4 separated fractions, including H₂O fraction, 30% MeOH/H₂O fractions, 50% MeOH/H₂O fraction, and 100% MeOH. The 100% MeOH fraction was further chromatographed on Lobar RP-18 to get purified ferulic acid.