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. Author manuscript; available in PMC: 2012 Jul 24.
Published in final edited form as: Curr Protoc Microbiol. 2009 Nov;CHAPTER:Unit–15C.3. doi: 10.1002/9780471729259.mc15c03s15

Figure 2. Cartoon of two types of RV particles in a CsCl gradient.

Figure 2

Following centrifugation of RV in a CsCl gradient, two distinct, cloudy, whitish-colored bands will be visible. Shining a light on the gradient will facilitate visualization of the bands. The upper band consists of infectious, triple-layered particles (TLPs; density ~1.36 g/cm3) and the lower band consists of non-infectious, double-layered particles (DLPs; density ~1.38 g/cm3). Harvest the upper band as a source of purified RV.