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. 2010 Jan 13;30(2):670–684. doi: 10.1523/JNEUROSCI.4656-09.2010

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Copyright © 2010 the authors 0270-6474/10/300670-15$15.00/0

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Figure 4. — Verification of 5HT neuron-enriched gene expression. A, B, Taqman RT-PCR verified 5HT neuron-enriched expression of transcription factors Rnf112, Zcchc12, Tox and RIKEN genes 2010204K13Rik, AW551984, and 1700042O10Rik. C, Double-label immunohistochemistry for EYFP (brown) and in situ hybridization for AW551984 transcript (purple) verified the enriched expression of est AW551984 in 5HT neurons. D–F, Coimmunohistochemical staining of E12.5 neural tube confirmed FoxP1 (E) expression in YFP⁺ (D) 5HT neurons (F, overlay). Scale bars: C, 40 μm; D–F, 100 μm.