Table 2.

Gene ontology and pathway enrichment in R⁺C⁺ 5HT neurons

Code	Gene set name	Size	R+ Bon	C+ Bon
Biological process-enriched gene sets
310	Lipid metabolic process	195/271	0.002807	8.72 × 10−6
125	Ceramide metabolic process	7/10	0.011107	0.003837
62	Calcium ion transport	12/24	0.017271	2.07 × 10−5
765	Sphingolipid metabolic process	12/24	0.041981	0.0021
573	Proteolysis	78/162	1.87 × 10−5	3.94 × 10−9
561	Protein processing	19/42	0.03184	0.01963
335	Membrane lipid metabolic process	36/88	0.008563	0.000265
246	G-protein-coupled receptor protein signaling pathway	101/290	0.000209	0.003268
557	Protein modification process	214/567	2.02 × 10−7	5.86 × 10−6
456	Peptide metabolic process	5/9	7.1 × 10−6	0.682079
763	Sphingoid metabolic process	9/11	0.055297	0.015825
168	Di-, trivalent inorganic cation transport	17/29	0.152911	0.000429
86	Cellular lipid metabolic process	116/212	0.05149	0.001441
338	Metal ion transport	53/102	1	0.00036
74	Cation transport	62/128	0.138503	7.68 × 10−5
555	Protein metabolic process	514/1066	0.134745	0.000225
296	Ion transport	75/161	1	0.001533
Cellular component-enriched gene sets
232	Voltage-gated calcium channel complex	12/15	1.49 × 10−5	0.04144
103	Intrinsic to membrane	387/1169	2.76 × 10−11	3.1 × 10−13
92	Integral to plasma membrane	275/846	2.79 × 10−5	7.08 × 10−6
193	Protein complex	176/703	6.86 × 10−7	1.28 × 10−6
Molecular function-enriched gene sets
393	Voltage-gated channel activity	32/66	0.000161	5.47 × 10−6
102	Exopeptidase activity	13/28	0.001926	5.75 × 10−5
30	ATPase activity coupled to transmembrane movement of ions	10/22	0.041303	0.03688
159	Ion channel activity	57/126	0.000252	7.01 × 10−9
160	Ion transmembrane transporter activity	94/245	0.011539	2.22 × 10−7
50	Carboxypeptidase activity	4/12	0.033525	0.041814
201	Neuropeptide hormone activity	5/10	0.016866	1
18	Aminopeptidase activity	9/10	1	0.000943
314	Serine-type peptidase activity	26/41	0.277263	2.87 × 10−5
312	Serine-type endopeptidase activity	22/37	0.889975	8.5 × 10−5
55	Cation transmembrane transporter activity	100/194	0.053817	3.06 × 10−6
Canonical pathway-enriched gene sets
509	RAB pathway	9/10	6.33 × 10−7	0.000801
454	Nos1 pathway	15/20	0.001063	0.00845
249	HSA00600 sphingolipid metabolism	18/31	0.00138	0.022235
244	HSA00564 glycerophospholipid metabolism	32/68	0.002839	0.000696

Bon, Bonferroni-corrected p value.