Figure 10.

MeCP2–e2 neurotoxicity is inhibited by FoxG1. A, Viability of neurons transfected with GFP, MeCP2–e2-Myc, and MeCP2–e2-del9-Myc and treated with HK for 24 h. Viability of neurons was normalized to CGNs transfected with GFP and treated with HK. MeCP2–e2-del9 killed healthy neurons more than MeCP2–e2. B, Viability of neurons transfected with GFP, FoxG1-1–191-Flag, and FoxG1-1–233- Flag and treated with HK for 24 h. Viability of neurons was normalized to CGNs transfected with GFP and treated with HK. Both the deleted constructs of FoxG1 killed neurons. C, RT-PCR analysis of HT22 cells transfected with FoxG1, GFP, and MeCP2–e2. Overexpression of FoxG1 did not affect endogenous mRNA levels of Mecp2, and similarly overexpression of MeCP2–e2 did not affect the endogenous mRNA levels of FoxG1.