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. 2012 Feb 22;32(8):2846–2855. doi: 10.1523/JNEUROSCI.5841-11.2012

Figure 7.

Figure 7.

Analysis of interaction between FoxG1 and MeCP2–e2. A, Schematic representation of the deletion constructs of FoxG1 used in this study. CS, Conserved sequence; DBD, DNA binding domain; TRD, transcriptional repression domain. B, Coimmunoprecipitation analysis of deletion constructs of FoxG1 (FoxG1-37–481, FoxG1-172–481, FoxG1-1–275, and FoxG1-1–336) and MeCP2–e2-GFP. The immunoprecipitation (IP) was performed with Flag antibody and immunoblotting (IB) was performed with GFP antibody. C, Coimmunoprecipitation analysis of deletion constructs of FoxG1 (FoxG1-1–191, FoxG1-1–233,and FoxG1-1–254) and MeCP2–e2-Myc. The immunoprecipitation was performed with Flag antibody and immunoblotting was performed with Myc antibody. FoxG1-1–191 and FoxG1-1–233 do not interact with MeCP2–e2.